Journal: Chembiochem : a European journal of chemical biology

Article Title: Reaction Pathways for the Enzymatic Degradation of Poly(Ethylene Terephthalate): What Characterizes an Efficient PET-Hydrolase?

doi: 10.1002/cbic.202200516

Figure Lengend Snippet: Figure 2. Progress curves for the hydrolysis of nanoPET by LCC, IsP, HiC, and TfC. Panels A and B show respectively the concentration of protons measured by the pH-stat to maintain the pH at (pH 8.0) and the total concentration of soluble fragments in terephthalic acid equivalents, CTeq (see Eq. 2). On the right ordinate, these functions are expressed in relative amounts as defined in Eqs. (1) and (3). Error bars represent the standard deviation of duplicate measurements.

Article Snippet: T and ETE were purchased from Sigma-Aldrich, while ET, and the di- and tri-aromatic fragments (TET, TETE and TETET) were synthesized in-house starting from the mono-tBu ester of terephthalic acid (tBu-T-OH, CAS 20576-82-3). tBu T-OH was purchased from Enamine Ltd (NJ, USA).

Techniques: Concentration Assay, Standard Deviation

Journal: Chembiochem : a European journal of chemical biology

Article Title: Reaction Pathways for the Enzymatic Degradation of Poly(Ethylene Terephthalate): What Characterizes an Efficient PET-Hydrolase?

doi: 10.1002/cbic.202200516

Figure Lengend Snippet: Figure 3. Comparison of proton release and product formation. Total concentration of soluble fragments in terephthalic acid equivalents, CTeq (see Eq. 2) plotted as a function of proton release. The results are derived from progress curves measured by pH-stat- and RP-HPLC analysis. Error bars represent the standard deviation of duplicate measurements.

Article Snippet: T and ETE were purchased from Sigma-Aldrich, while ET, and the di- and tri-aromatic fragments (TET, TETE and TETET) were synthesized in-house starting from the mono-tBu ester of terephthalic acid (tBu-T-OH, CAS 20576-82-3). tBu T-OH was purchased from Enamine Ltd (NJ, USA).

Techniques: Comparison, Concentration Assay, Derivative Assay, Standard Deviation

Journal: Chembiochem : a European journal of chemical biology

Article Title: Reaction Pathways for the Enzymatic Degradation of Poly(Ethylene Terephthalate): What Characterizes an Efficient PET-Hydrolase?

doi: 10.1002/cbic.202200516

Figure Lengend Snippet: Figure 5. Results for the stochastic model fitted to experimental results for LCC (top panels) and HiC (lower panels). Average values and standard deviation of 5 repetitions of the stochastic models are shown. A/B) Values corresponding to terephthalic acid equivalents CTeq (see Eq. 2) occurring in each species during the reaction. C/D) Mode of hydrolytic event occurring over the time course of the stochastic model. E/F) Accumulated number of cuts over the whole simulation for each of the four reaction types defined in the model (i.e., endolytic, exolytic, bulk (homogenous catalysis) and autohydrolysis (non-enzymatic hydrolysis).

Article Snippet: T and ETE were purchased from Sigma-Aldrich, while ET, and the di- and tri-aromatic fragments (TET, TETE and TETET) were synthesized in-house starting from the mono-tBu ester of terephthalic acid (tBu-T-OH, CAS 20576-82-3). tBu T-OH was purchased from Enamine Ltd (NJ, USA).

Techniques: Standard Deviation

Journal: Chembiochem : a European journal of chemical biology

Article Title: Reaction Pathways for the Enzymatic Degradation of Poly(Ethylene Terephthalate): What Characterizes an Efficient PET-Hydrolase?

doi: 10.1002/cbic.202200516

Figure Lengend Snippet: Figure 6. Reaction pathway For LCC of soluble products. Sankey plot for LCC, illustrating the degradation pathway from insoluble PET towards monomers over the reaction course elucidated by the stochastic model. Different modes of action and soluble PET fragments are highlighted. The flux of terephthalic acid equivalents is scaled to the relative strength of the depicted linkage, and the percentages indicate the fraction of all terephthalic acid equivalents flowing through a given node. For the current discussion, it is important to note that soluble tri-aromatic (TETETE and TETET) and di-aromatic (TET, TETE and ETETE) compounds are very common intermediates. About 70% of all mono-aromatic products that eventually formed passed through one of these intermediates.

Article Snippet: T and ETE were purchased from Sigma-Aldrich, while ET, and the di- and tri-aromatic fragments (TET, TETE and TETET) were synthesized in-house starting from the mono-tBu ester of terephthalic acid (tBu-T-OH, CAS 20576-82-3). tBu T-OH was purchased from Enamine Ltd (NJ, USA).

Techniques: